| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.010560 |
| Chromosome: | chromosome 10 |
| Location: | 2190081 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801121 | (1 of 1) PTHR10459:SF7 - DNA LIGASE 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACTACACGTCATTGTCATCATCATTACGCAGGTGAAGCCGGACTACG |
| Internal bar code: | CGATTAGGTCCTTTTGGTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACAGTGTTGTCCGTGC |
| Suggested primer 2: | GGCATAGTGGTGAAGCTGGT |