Insertion junction: CLIP2.010569_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.010569
Chromosome:chromosome 16
Location:4984210
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686250 (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCCGGCGAAATTCCCGACGCGGTTGCAACGCGTAAAACGCTCTGCTGG
Internal bar code:GATATCAAATTATTATCAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1872
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGAAAGGCCGGATCACC
Suggested primer 2:CTGTTCACGGACTGATGCCT