| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.010572 |
| Chromosome: | chromosome 16 |
| Location: | 1632628 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654300 | NDK5,RSP23 | (1 of 1) PF00334//PF00612//PF05186 - Nucleoside diphosphate kinase (NDK) // IQ calmodulin-binding motif (IQ) // Dpy-30 motif (Dpy-30); Radial Spoke Protein 23 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCAGCGGGCCTATCTGGGCGCTTGTGCTGGCCAAGCCTGGTGCCATT |
| Internal bar code: | TATACGGTGGGATGTTGTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2258 |
| LEAP-Seq percent confirming: | 97.6744 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCGTGGGGAAGAAGAA |
| Suggested primer 2: | CTATCTGAGTTCGGTCCCGC |