| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010576 |
| Chromosome: | chromosome 1 |
| Location: | 5181453 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036200 | (1 of 1) PF01477//PF07228 - PLAT/LH2 domain (PLAT) // Stage II sporulation protein E (SpoIIE) (SpoIIE) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAGCGCGGGCAGCTACTTGCAGCCAACCTGGGCGACAGCGGCTGCCT |
| Internal bar code: | CATCTGGTATAAACGCTATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3530 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATGTGGCCCTGACAGAT |
| Suggested primer 2: | TCCATACTGCACCATACCGC |