Insertion junction: CLIP2.010595_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.010595
Chromosome:chromosome 1
Location:6205253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g044250 CAM4 Calmodulin-like protein; (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGACAAGCTCCATCCGGCAGTGACACGCGTCATGTGAGTGCGGCGATG
Internal bar code:GCATACGCTTTGAAGATCTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2471
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATCCCAGCCGATGACTC
Suggested primer 2:ACACCAAGGTTAGGCGTCAG