| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.010619 |
| Chromosome: | chromosome 3 |
| Location: | 683963 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145767 | RRP6,RRP6a | putative exosome subunit; (1 of 1) K12951 - cation-transporting P-type ATPase D (ctpD) | 3'UTR |
| Cre03.g145787 | HSP22C | (1 of 1) IPR000104//IPR002068//IPR008978//IPR031107 - Antifreeze protein, type I // Alpha crystallin/Hsp20 domain // HSP20-like chaperone // Small heat shock protein HSP20; Heat shock protein 22C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGACCGCCACCTCACCGCTGAGCTGGCCAACGTCTTTGGCGACTCGCC |
| Internal bar code: | CGCAGTTTTATTACTTATAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3743 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGATGTGCAGGTCCGATT |
| Suggested primer 2: | ACCCTTCTCCGTTTACGCAG |