Insertion junction: CLIP2.010638_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010638
Chromosome:chromosome 17
Location:3816393
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g727201 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGAGAGGCTTCAACACTTGACAGCCATGGCGCGCGCTGCTGCTGCCG
Internal bar code:CAAGGCCTTCGTCGATTGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:6095
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTCTGTTCGCGGCTGTAT
Suggested primer 2:CTTCGTCATGCGTGTTAGCG