| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010640 |
| Chromosome: | chromosome 7 |
| Location: | 3525331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGACTGTAGGGTCGGTATCGGCGCGCGGTTTGTTGCGGACCGGCAGC |
| Internal bar code: | GTCGCAAGGGTGGGGGTATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGAAAAATGTGCGCTGT |
| Suggested primer 2: | GTTGGGGTTTTCACGTGACG |