| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.010667 |
| Chromosome: | chromosome 4 |
| Location: | 24824 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213761 | (1 of 1) PTHR31793//PTHR31793:SF5 - FAMILY NOT NAMED // THIOESTERASE-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTCCGTGTCACAGTGGCGGTGGCCAAGGTACGTCATAATAGGTGCCA |
| Internal bar code: | CTCACTTCAGGGTAATTGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2702 |
| LEAP-Seq percent confirming: | 92.4242 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCCGACATGTAGCCTG |
| Suggested primer 2: | CCTCAGGTAATGGCGGTTGT |