Insertion junction: CLIP2.010675_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.010675
Chromosome:chromosome 11
Location:2549264
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095097 FKB21,FKB17-2,FKB17B (1 of 2) PTHR10516:SF290 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE; peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGGCAAGGAGCACTGCAGCTGCATAAGCGGGTCGTTGTACATCACC
Internal bar code:GATGCTGGGCGCCACAATGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1155
LEAP-Seq percent confirming:92.5926
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCTGTGTCTGCTCCAAT
Suggested primer 2:AACACTTTTTCGTCACCGCG