| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.010676 |
| Chromosome: | chromosome 3 |
| Location: | 5535116 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185050 | FAP53,CFAP53 | Flagellar Associated Protein 53; (1 of 1) PTHR31183:SF1 - COILED-COIL DOMAIN-CONTAINING PROTEIN 11 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGCCACATGCGCTTCTCCTCGGCCTCCATGATCCGCTGCGCCATCT |
| Internal bar code: | TAAATGAGGACTACCTCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1359 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCGGGCCATACTTAGG |
| Suggested primer 2: | ATCTTCAGGTCCAGCTCCCT |