| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.010677 |
| Chromosome: | chromosome 17 |
| Location: | 6452832 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744197 | (1 of 1) IPR000477//IPR002156//IPR010998//IPR011010//IPR012337//IPR013762//IPR023109 - Reverse transcriptase domain // Ribonuclease H domain // Integrase, Lambda-type, N-terminal // DNA breaking-rejoining enzyme, catalytic core // Ribonuclease H-like domain // Integrase-like, catalytic domain // Integrase/recombinase, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGATCTGGGTTATAGGCCTCGGTCATGCCCTTGGACAGCAGGTCGTCG |
| Internal bar code: | TCATTGGGATGTCCGTTATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1328 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAAGTTAGGGGGCGCCTG |
| Suggested primer 2: | GATACGCATCTCCATCCGCA |