| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010695 |
| Chromosome: | chromosome 7 |
| Location: | 4594189 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344200 | (1 of 3) IPR000104//IPR020846 - Antifreeze protein, type I // Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGGTGCTGGCGTCATGAGTGATGTCAGGGGGTGGTGTGGGGGATCGC |
| Internal bar code: | TTTGTCTGCATCAGTAGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 845 |
| LEAP-Seq percent confirming: | 86.1111 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTATGCGATGCGTGTG |
| Suggested primer 2: | TACGTCGGAGCGATAACGTG |