| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.010697 |
| Chromosome: | chromosome 6 |
| Location: | 3464083 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278107 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGTCCTGACAAGAGCGAGGCGCACCTGCTCCACGCCCACGTGGACCCC |
| Internal bar code: | GGTTTACGTCTGTTTGTGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1480 |
| LEAP-Seq percent confirming: | 54.1667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAGGAAGCTTGCAGCAG |
| Suggested primer 2: | GTCCAAGCTACCCCAACCTC |