Insertion junction: CLIP2.010713_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010713
Chromosome:chromosome 2
Location:403134
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g075800 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCAAGCACACACCCGCGAGCAACTACATGGCCACACACACCCGACTGC
Internal bar code:TACAGTGAGGGACGCTTAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2536
LEAP-Seq percent confirming:95.8904
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGTACAGCACAACAGAGT
Suggested primer 2:AAGGTGTGTACGCTGGGATG