Insertion junction: CLIP2.010713_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010713
Chromosome:chromosome 13
Location:945664
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g568100 (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGGACACACAACACACAGACAACACAATCACAAGCAACAGACAACCAC
Internal bar code:GCGTCGTACATTGTTTGGGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2633
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCTGCTGTAGCTGTGCCT
Suggested primer 2:GACACCTGGGAAAGAGGACG