Insertion junction: CLIP2.010714_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.010714
Chromosome:chromosome 11
Location:3694817
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g478750 FKB2,FKB15-2,FKB15B Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516//PTHR10516:SF305 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCGCTTTATCTGGCCCCCCGCTTTTACAAGGCACTACTGGAGACGCA
Internal bar code:AAATCACGCGTGCCTGAAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1581
LEAP-Seq percent confirming:97.7273
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCTATCCCCCAGTCCCA
Suggested primer 2:CTGGTGTTCGATGTGGAGCT