| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010723 |
| Chromosome: | chromosome 13 |
| Location: | 1130796 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569500 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTCACCTGACGCCCGAGCACATCGCCGAGCTTGATGCAGCCGTGGAC |
| Internal bar code: | AAGATGGTAAGTATTGCAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1877 |
| LEAP-Seq percent confirming: | 67.5 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCACGAAGAGACAACCA |
| Suggested primer 2: | CGCATGTATGAACGGGCAAG |