Insertion junction: CLIP2.010728_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010728
Chromosome:chromosome 7
Location:5382091
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350350 FAP217 Coiled-Coil Flagellar Associated Protein 217 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGCGGGTCGCAGGTGACGACTGGGCACCGGTATGGCCAACTCAACTG
Internal bar code:GACTTTTTCTTGGTAGGAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2162
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCGTCATCCTCGAATACA
Suggested primer 2:AGTGGGGAATAAGCAAGGGC