| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010729 |
| Chromosome: | chromosome 3 |
| Location: | 6270461 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191800 | (1 of 33) PF13855 - Leucine rich repeat (LRR_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGTCAGTGCGTGGGCCCAGTGGTACCAGGAAAGTCAACAGGTACGG |
| Internal bar code: | GGTGATCCGTGGTCGGACCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGTGGTATGTAGCTGTT |
| Suggested primer 2: | AGCAATCAAGAGTCCCACCG |