| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.010740 |
| Chromosome: | chromosome 2 |
| Location: | 6544942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389100 | GOT1 | (1 of 3) PF04178 - Got1/Sft2-like family (Got1); Got1, COPII vesicle component | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGATGGGGGCCGAGGCGTCGTGGAGTAGCAGGGCGAGCGGCGCCCGG |
| Internal bar code: | CTCAACGGATCTTTACTCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2348 |
| LEAP-Seq percent confirming: | 92.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTATGCGCCCCAAATG |
| Suggested primer 2: | TGATCCTCGAAGCATACGGC |