| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.010756 |
| Chromosome: | chromosome 3 |
| Location: | 6022828 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189750 | TIM8 | (1 of 1) K17780 - mitochondrial import inner membrane translocase subunit TIM8 (TIM8); Translocase inner membrane subunit 8 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTCCGGGGTTACATGGCAGGGGGTTGGCGAGGTGGATGTGGATGACG |
| Internal bar code: | AGTTCGCAAATATCACACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2476 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGACCCTCACCCTCCTA |
| Suggested primer 2: | GTGGCTGTATACGGACCTCG |