Insertion junction: CLIP2.010771_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010771
Chromosome:chromosome 14
Location:603100
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g611850 (1 of 1) K17087 - transmembrane 9 superfamily member 3 (TM9SF3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCCCCTCACCAACCCCACTCCAGCTTCAACAACCCCTATCCTGCGG
Internal bar code:ATGAGGTCCGTGGTTGTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:481
LEAP-Seq percent confirming:89.4737
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGTGTGTTCGGTCA
Suggested primer 2:AGACGCCCAAACCATCCATT