| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.010778 |
| Chromosome: | chromosome 13 |
| Location: | 1375613 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571750 | (1 of 1) PTHR10050 - DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTTCCACAGCTTCCCCTTGCCCTCCCACTCCAGAACCCAGTTGTCAC |
| Internal bar code: | AATAATATGGGGGGGAATGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1595 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGCCGGAGATTTGAGTG |
| Suggested primer 2: | TCGCAGTAGTGTCACCAACC |