| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010782 |
| Chromosome: | chromosome 13 |
| Location: | 1350503 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571500 | CST2 | Chlamydomonas-specific membrane transporter of unknown function | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGTAGAGTGCGAAAGCATAAAGATCGGTTGCGCAGTGTTTTCGCCG |
| Internal bar code: | ATTAAGGTTTCATAAATTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2189 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCAGCTTGTCACTCCCG |
| Suggested primer 2: | GCAAAGGGACAAAAGGGCAG |