Insertion junction: CLIP2.010798_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010798
Chromosome:chromosome 12
Location:1468558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g488150 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCCAGAAATGGACGCTGGAGGCGACACTACGCTTGCAACTGCATAGC
Internal bar code:GGATGTTTAAGCTGACATCATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2095
LEAP-Seq percent confirming:39.759
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:50
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCTGCTCACCTTCCATGC
Suggested primer 2:TCATGGTTTCGTGTGCTCGA