Insertion junction: CLIP2.010803_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010803
Chromosome:chromosome 4
Location:2956737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTGCTGTCTTCAATCGTCGGGTGCGGTCAACTTGCGCTTTGTCGACGC
Internal bar code:ATAGGCCTCATCGAGAGCCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2098
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCGTTGTTGTGCACAAG
Suggested primer 2:CGGCAGCTGCAATGGAATAC