Insertion junction: CLIP2.010804_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.010804
Chromosome:chromosome 12
Location:5902656
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533600 DESI6,DeSI-6 (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAGGGCCCGCGGCCCTGACTCTTAGTCCCATGTATCATCCAGTAGTCC
Internal bar code:CTAGCTGGTGTACTGAACGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1925
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAATCGTTACAACTCGCC
Suggested primer 2:GAACTGTGGCCCTGAATGGA