| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010826 |
| Chromosome: | chromosome 3 |
| Location: | 344009 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144607 | (1 of 79) IPR011992 - EF-hand domain pair | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAAAAAAACACTGTCACACTGTCTTTGCGCAACGTTTTCCTTGTGCTC |
| Internal bar code: | CTGTACTGTCATCATAGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 197 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGAGTCGCACTTTGTGG |
| Suggested primer 2: | GAGCAGACACAGTCGACACA |