Insertion junction: CLIP2.010830_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010830
Chromosome:chromosome 5
Location:3552025
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g233750 (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTACTCCTCTTCCCACTCCTCACGCCCACCTCTTGACGAGCACCTTCG
Internal bar code:TCAATGAATCTTCGTAAGAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1018
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGGGTTGGTGTTGACGA
Suggested primer 2:CGCTCTCCTTCCACTCCATC