| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.010835 |
| Chromosome: | chromosome 2 |
| Location: | 4422630 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105900 | (1 of 1) 3.1.1.7 - Acetylcholinesterase / True cholinesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGACCGCCATGGCGTCCGCACTGCCGCTGAATGACACGGCGGAGGAAA |
| Internal bar code: | CAGTTGGTTATTACGCTCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4879 |
| LEAP-Seq percent confirming: | 98.374 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCGGGGTTACTAGCTAG |
| Suggested primer 2: | TGTCCAGGCTCACGTTCTTC |