| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.010835 |
| Chromosome: | chromosome 2 |
| Location: | 4422718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105900 | (1 of 1) 3.1.1.7 - Acetylcholinesterase / True cholinesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCAGTAATGCGGACTTGGCCGTTTATGGCGGGTCAATCAAGGGCCA |
| Internal bar code: | TTGGTCCCGATTATAGATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3242 |
| LEAP-Seq percent confirming: | 98.8372 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCAGGCTCACGTTCTTC |
| Suggested primer 2: | GCAGCGGGGTTACTAGCTAG |