Insertion junction: CLIP2.010857_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.010857
Chromosome:chromosome 13
Location:2481043
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g579734 GLC1 (1 of 1) 3.2.1.106 - Mannosyl-oligosaccharide glucosidase / Processing A-glucosidase I; Glucosidase 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCTCTCTCTCTCTCTCACTGTCTTTGCGCAACGTTCTCCTTGTGCTC
Internal bar code:CATTGTTTGGGTTAAAGGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:752
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TATTTACAACCCACCCCGGC
Suggested primer 2:CACCACAGACACACCCCTAC