| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.010860 |
| Chromosome: | chromosome 11 |
| Location: | 663726 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467616 | Dyf-13,IFT56,Ttc26 | (1 of 3) PF12895 - Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3); Intraflagellar Transport Protein 56 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACAGGTCGGGAGGGGTGCAAGACTGGCAGAGACTGACAAGCTCTGCA |
| Internal bar code: | ATAACTAACAGAGAGTGATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 894 |
| LEAP-Seq percent confirming: | 44.8276 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAGGTAGGTGGAGATGT |
| Suggested primer 2: | TACTCGACCGTTATGAGCGC |