| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010873 |
| Chromosome: | chromosome 3 |
| Location: | 4595531 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176600 | FAP374 | Flagellar Associated Protein 374 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATGCATTGTCTACGCCGACAGCTGCCACTCGCCCCTTACCCCGGCCC |
| Internal bar code: | GCTATAATCAGCGTTATACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 6.42857 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGTCCGTGACGTTGAGG |
| Suggested primer 2: | CGTCGGATGGGATTGTCGAT |