Insertion junction: CLIP2.010879_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.010879
Chromosome:chromosome 12
Location:8165070
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g552001 (1 of 4) PF01435 - Peptidase family M48 (Peptidase_M48) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGTTGCCTTCCGGGGTTCGTGGGTTTGGAATCTAGCTCACACTGCGC
Internal bar code:AAACCGCAAACTAGTTTTTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:92.1053
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CAAATGACCCTCCCTCCCAC
Suggested primer 2:ACTCCCCAACAGCAACAACA