Insertion junction: CLIP2.010882_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010882
Chromosome:chromosome 1
Location:4578291
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g031500 (1 of 1) 1.10.3.2//1.10.3.3 - Laccase / Urishiol oxidase // L-ascorbate oxidase / Ascorbase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGATGCTGCGCCTGCACACACATACACGCACCACACCGCCACGTCGCC
Internal bar code:CTGTTTGGGGCCGCTAAATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2057
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CTCATCGCCGTCATAGGACC
Suggested primer 2:CGACCCCAATTTATGCGCTG