Insertion junction: CLIP2.010892_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.010892
Chromosome:chromosome 13
Location:2141535
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577550 DDB2 (1 of 1) K10140 - DNA damage-binding protein 2 (DDB2); UV-damaged DNA binding complex subunit 2 protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACACGCCAACCCGCCCGGCACCGCCGAAATCCCCCCTCCCAAAAACTG
Internal bar code:GACTTTTGAATCCGTAAAACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:437
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCTCTGGTACGCGAGAT
Suggested primer 2:CGCATATTCGACATCCGGGA