Insertion junction: CLIP2.010896_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010896
Chromosome:chromosome 16
Location:4193443
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGCTGCAGCCGCGGACTGCTCCGATATCGCGCCCAACCCCGCCAAAT
Internal bar code:ATGTTGGTCCGTGGTAGCGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2557
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCATACATGGTACGACCG
Suggested primer 2:GGCCTCCTCTGCTTCAGTAC