Insertion junction: CLIP2.010899_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010899
Chromosome:chromosome 9
Location:2064909
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396450 APT3,GOX15 Acid phosphatase; (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCTCGCCCCGTGGCCTCGCCAGCTCCACCGCCCGCGCTCTTGTGGAC
Internal bar code:GTTGTCACAGTTCTCGAACGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1755
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGCGTGTTGAAGACCGTC
Suggested primer 2:GACCCACCCTCCTTGTCTTG