| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010917 |
| Chromosome: | chromosome 16 |
| Location: | 4542600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671400 | ARS1 | (1 of 2) IPR000917//IPR012083//IPR017850 - Sulfatase // Arylsulfatase // Alkaline-phosphatase-like, core domain; Periplasmic arylsulfatase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGTGAGTCATCGCAGCGCAGTGGTCGGTGTCCCAAGCAACAGCGTCC |
| Internal bar code: | GGGCTAGGTCTGGCCGGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1298 |
| LEAP-Seq percent confirming: | 70.1754 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTACCCATTCCACCCTA |
| Suggested primer 2: | ACGCTGTCAAGGGATCGTTT |