Insertion junction: CLIP2.010940_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.010940
Chromosome:chromosome 6
Location:6168124
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291850 (1 of 6) PTHR24114:SF2 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN 74 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTAACGCATCAACAGAGACGTGGCCGCCCAAACCCCTTTTCCGCCTT
Internal bar code:CTTGTTAGCCATTTAGTTGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:876
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GACAATGGAATCTTCGCCGC
Suggested primer 2:GGGCAAGAAACGGGGGATTA