Insertion junction: CLIP2.010965_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.010965
Chromosome:chromosome 7
Location:916049
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g318950 (1 of 1) IPR000008//IPR000104//IPR003072 - C2 domain // Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTCAGGTGGAGGGTCCCTGCATGCCGCCCCAGCGGCGGCGGCGGCGGC
Internal bar code:AACTCCTGTGGGAAATTATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1884
LEAP-Seq percent confirming:89.1892
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCGACCCAACATTCATCA
Suggested primer 2:AATGGTAGGGCAAAAGCGGA