Insertion junction: CLIP2.010969_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.010969
Chromosome:plastome
Location:79413
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802297 psbB,2717002,ChreCp032 (1 of 1) K02704 - photosystem II CP47 chlorophyll apoprotein (psbB); photosystem II P680 chlorophyll A apoprotein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGATGATGATATTAATGACCAAGTTGAATTCGGTAAATACAAAAAACTT
Internal bar code:AGGTCTTGTACCTGGTCTCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:988
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGATGCACCACCAACTC
Suggested primer 2:GACCCATACAACCCAGGTGG