| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.010970 |
| Chromosome: | chromosome 4 |
| Location: | 3503752 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g228000 | (1 of 1) PF13890 - Rab3 GTPase-activating protein catalytic subunit (Rab3-GTPase_cat) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCCTTCACGGTGATGCTGTCCACGCTGCTGACCGGCCGCAGCCTGGC |
| Internal bar code: | AGCTGGTACGCATGTCCTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 531 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGGGGTGTTTTGAGGG |
| Suggested primer 2: | TGCGTACAGAGCATGTGTGT |