| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.010982 |
| Chromosome: | chromosome 1 |
| Location: | 8122479 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g065822 | FAP80,IFT122 | (1 of 54) IPR001680//IPR017986 - WD40 repeat // WD40-repeat-containing domain; Intraflagellar Transport Protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGTCAATTGACTGAAATCCCTTGCCGTCGATCTTGCCCTGCACCCAG |
| Internal bar code: | GTATCTGGAAAGTTCTTAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3070 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGATCTCGTTCTGGTCC |
| Suggested primer 2: | CTATCAGCTGGCCATGGAGG |