| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011010 |
| Chromosome: | chromosome 3 |
| Location: | 1166168 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149300 | (1 of 1) 2.8.4.5 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase / Methylthio-threonylcarbamoyl-adenosine transferase B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCGCCTGGCTGGGCCCCTTGACCGTGCAGCTGTTGATGAGCCACAGG |
| Internal bar code: | CTCACACGACTCGAGTCCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1838 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTCGCCTTTGGTTGGA |
| Suggested primer 2: | CCAGTGGAGATGGGCACAAT |