Insertion junction: CLIP2.011014_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011014
Chromosome:chromosome 6
Location:8232600
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g306550 (1 of 1) K01166 - ribonuclease T2 [EC:3.1.27.1] (E3.1.27.1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCCCAGCAAGTCCAACACCTACAAGGTGTCGGAGCTGGCGGACGCGG
Internal bar code:CTCATATTGTTTTAGAAGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1343
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACACACATCCACACCTG
Suggested primer 2:ATCTCTTCTCCTCCCCCACC