| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011030 |
| Chromosome: | chromosome 16 |
| Location: | 7074461 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674964 | (1 of 1) K06911 - uncharacterized protein (K06911) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGGCACCGGGGCGCTGCGCAACCTCGACCCCTACCTCATGCTGTGAG |
| Internal bar code: | AGTTTTGATGGAGGTCTGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTACGTTGAAGTGTCCCA |
| Suggested primer 2: | CCTGCACCCAAAGCTATGGA |