Insertion junction: CLIP2.011042_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011042
Chromosome:chromosome 11
Location:3456169
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTCGCGACCTCGCGACAGTCAAATGTCCATGGTCACGCGAGGTCGCTT
Internal bar code:AAACACAGGATTTTTAATTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:4.54545
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTTCCCCATCTACCAGA
Suggested primer 2:CAGCACCCCAGAGTATAGCG